IFATS

Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype.

admin — Wed, 29 Sep 2010 11:15:15 +0000

The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well as for their application in plastic or reconstructive surgery. Adipose-derived stem cells (ASCs) provide …

Authors: Estes BT; Diekman BO; Gimble JM; Guilak F
Institution: Departments of Surgery and Biomedical Engineering, Duke University Medical Center, Durham, North Carolina, USA.

Isolating adipose-derived mesenchymal stem cells from lipoaspirate blood and saline fraction.

admin — Mon, 27 Sep 2010 12:21:33 +0000

Isolation of adipose-derived stem cells (ASCs) typically involves 8+ hours of intense effort, requiring specialized equipment and reagents. Here, we present an improved technique for isolating viable populations of mesenchymal stem cells from lipoaspirate saline fractions within 30 minutes. Importantly, the cells exhibit remarkable similarities to those obtained using the traditional isolation protocols, in terms ...

Tissue engineering and the use of stem/progenitor cells for airway epithelium repair.

admin — Fri, 24 Sep 2010 11:17:04 +0000

Stem/progenitor cells can be used to repair defects in the airway wall, resulting from e.g., tumors, trauma, tissue reactions following long-time intubations, or diseases that are associated with epithelial damage. Several potential sources of cells for airway epithelium have been identified. These can be divided into two groups. The first group consists of endogenous progenitor ... Authors: Roomans GM Institution: School of Health and Medical Sciences, Orebro University, Orebro University Hospital, SE-70185 Orebro, Sweden. godfried.roomans@oru.se

Id4, a new candidate gene for senile osteoporosis, acts as a molecular switch promoting osteoblast differentiation.

admin — Wed, 22 Sep 2010 10:29:27 +0000

Excessive accumulation of bone marrow adipocytes observed in senile osteoporosis or age-related osteopenia is caused by the unbalanced differentiation of MSCs into bone marrow adipocytes or osteoblasts. Several transcription factors are known to regulate the balance between adipocyte and osteoblast differentiation. However, the molecular mechanisms that regulate the balance between adipocyte and osteoblast differentiation in ...

Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord.

admin — Mon, 20 Sep 2010 10:38:06 +0000

Up to 2.8 x 10(7) fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical cord (UC) using a simple and highly reproducible explant culture approach. Cells derived from whole UC, similar to cells collected ...